Axygen AxyMats AM-96-PCR-RD Silicone 96 Round Well Sealing Mat for PCR

Description

Product Overview

The Axygen AxyMats AM-96-PCR-RD silicone sealing mat is engineered to meet the rigorous demands of modern molecular biology laboratories. Constructed from high‑grade, food‑safe silicone, the mat delivers a flexible yet durable barrier that conforms precisely to the 96 round‑well format of standard PCR microplates. Each well is encircled by a continuous silicone ring that prevents evaporation, cross‑contamination, and sample spillage during thermal cycling, while still allowing rapid heat transfer for optimal reaction efficiency.

With dimensions of 9.2 x 7.3 x 4.5 inches and a lightweight profile of just 0.01 ounces, the mat is easy to store and handle. The product is supplied in a case of 50 mats, providing a cost‑effective solution for high‑throughput environments. Manufactured by Corning, a trusted name in laboratory glassware and consumables, the AM‑96‑PCR‑RD model carries the assurance of consistent quality control and compliance with industry standards.

Designed for non‑sterile applications, the mat is suitable for routine PCR workflows where sterility is not a critical requirement, such as standard amplification, genotyping, and cloning procedures. Its silicone composition is chemically inert, resisting degradation from common reagents, solvents, and temperature extremes encountered during typical PCR protocols.

Compatibility extends beyond standard 96‑well PCR plates to include a range of microplate formats that share the same well geometry, making the AxyMats mat a versatile addition to any lab inventory. The mat’s transparent appearance allows easy visual inspection of the underlying plate, ensuring that users can monitor reaction progress without removing the seal.

Usage

In a typical PCR setup, the sealing mat is placed directly onto the microplate after the reaction mixture has been dispensed into each well. The flexible silicone conforms to the plate surface, creating a tight seal that minimizes evaporation even during extended high‑temperature cycles. This is especially valuable for applications requiring precise volume control, such as quantitative PCR (qPCR) and high‑resolution melt analysis.

The mat is also well‑suited for use in thermal cyclers that lack a heated lid, as the silicone barrier helps maintain a stable internal environment while protecting the plate from external contaminants. Researchers performing multiplex PCR, endpoint detection, or downstream enzymatic reactions will appreciate the ease of removal—simply lift the mat away without leaving residue on the plate.

Because the mat is non‑sterile, it is ideal for laboratories that perform routine assays where sterility can be ensured through other means, such as using filtered tips and aseptic techniques. It is also compatible with automated liquid handling systems, allowing seamless integration into high‑throughput workflows without the need for manual placement.

Why Choose Us

Choosing the Axygen AxyMats sealing mat means selecting a product backed by Corning’s decades of experience in laboratory consumables. Each mat undergoes stringent quality inspections to guarantee uniform thickness, consistent elasticity, and reliable sealing performance across every unit in the case of 50.

Our commitment to customer satisfaction extends beyond product delivery. We provide responsive technical support to assist with integration into existing protocols, and we offer guidance on best practices for maximizing the lifespan of each silicone mat. Should any issues arise, our dedicated after‑sales team is prepared to address concerns promptly.

Environmental responsibility is also a core value. The silicone material is recyclable where local regulations permit, and the packaging is designed to minimize waste while protecting the product during shipping. By selecting this mat, laboratories not only improve experimental reliability but also contribute to sustainable laboratory practices.

Key Features

• High‑grade silicone provides a flexible, durable seal that prevents evaporation and cross‑contamination.
• Fits precisely around 96 round‑well PCR microplates, ensuring consistent thermal transfer.
• Lightweight and compact packaging (case of 50) supports high‑throughput laboratory workflows.
• Non‑sterile design suitable for routine PCR, qPCR, and multiplex assays with easy removal.
• Backed by Corning’s quality assurance and responsive customer support for reliable performance.

FAQ

Can the silicone mat be reused?

The mat is intended for single‑use applications to maintain optimal sealing performance. While the silicone material is durable, repeated use can lead to loss of elasticity and potential contamination.

Is the mat compatible with heated‑lid thermal cyclers?

Yes, the silicone seal works effectively with both heated‑lid and non‑heated‑lid cyclers. It provides an additional barrier that helps maintain consistent temperature across all wells.

What temperature range can the silicone withstand?

The silicone used in the AxyMats mat is rated for temperatures up to 250 °C, far exceeding the typical 95 °C denaturation step in PCR, ensuring it will not degrade during standard cycling protocols.

How should the mat be disposed of after use?

Because the mat is made of recyclable silicone, it can be placed in a recycling stream where local regulations allow. If recycling is not available, dispose of it in regular waste following your institution’s safety guidelines.

Will the silicone interfere with fluorescence detection in qPCR?

No, the transparent silicone does not impede optical detection. It allows the excitation and emission light to pass through, making it suitable for real‑time PCR applications.